Glenda D. WebberOffice that BiotechnologyIowa State UniversityNorth central Regional Publication

Where the story began

For centuries, humans have actually searched for chop plants that can survive and also produce in spite of insect pests. Intentional or unknowingly, old farmers selected for pest resistance gene in your crops, sometimes by actions as basic as collecting particle from only the highest-yielding plants in their fields.

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With the arrival of genetic engineering, genes for insect resistance now deserve to be moved into plants more quickly and deliberately. Bt technology is only one example of methods genetic engineering may be supplied to construct insect-resistant crops now and in the future.

The Bt chapter

Bacillus thuringiensis, generally known together Bt, is a bacterium the occurs naturally in the soil. Because that years, bacteriologists have actually known that some strains the Bt produce proteins that kill details insects through alkaline digestive tracts. As soon as these insects ingest the protein created by Bt, the function of their digestive systems is disrupted, developing slow growth and, ultimately, death.

Bt is very selective — various strains of the bacter kill various insects and only those insects. Strains the Bt room effective versus European corn borers and cotton bollworms (Lepidoptera), Colorado potato beetles (Coleoptera), and certain flies and also mosquitoes (Diptera). Bt is not harmful to humans, various other mammals, birds, fish or advantageous insects.

Bt was first identified in 1911 when it was found that it killed the larvae the flour moths. Bt was registered as a biopesticide in the U.S. In 1961. Today it is offered in insecticide sprays offered to residence gardeners and others worldwide.

In 1983, the people Health Organization offered Bt in West Africa to regulate disease-carrying blackflies. In the U.S., miscellaneous strains of Bt are used to control spruce budworms and gypsy moths in forests, cabbage worms in broccoli and also cauliflower, loopers or budworms in cotton and also tobacco, and also leaf rollers in fruits.

However, much less than one percent of every pesticides supplied in the U.S. Annually contain Bt (Monsanto). Together an ingredient of advertisement sprays, Bt is fairly expensive and also has some drawbacks. Although some pesticides kill on contact, Bt need to be eaten by insects to be effective. Sunlight breaks down Bt, and also rain washes the from the plants. Therefore, Bt need to be applied exactly where and when the target insects space feeding, and also they have to consume the quickly prior to it disappears.

Bt and biotechnology

Today, plants have the right to be gene engineered to create their own Bt. Hereditary (recombinant DNA) design is the change of DNA molecules to produce alters in plants, animals or various other organisms. DNA (deoxyribonucleic acid) is a double-stranded molecule that is current in every cabinet of one organism and also contains the hereditary information that overcome from parents to offspring. This hereditary information is contained in individual units or sections of DNA referred to as genes. The gene that space passed from parent to offspring recognize the traits the the offspring will certainly have.

In the last twenty years, scientists made a surprising discovery — DNA is interchangeable among animals, plants, bacteria ... Any type of organism! In addition to utilizing traditional reproduction methods of improving plants and also animals v years of cross-breeding and selection, scientists can now isolation the gene or gene for the traits they want in one animal or plant and also move them right into another. The course, when a characteristics is controlled by several genes, the transfer procedure is much more difficult. The tree or animals modified in this means are dubbed transgenic.

Out of Bt ... Into the plants

DNA an innovation makes it feasible to locate the gene that produces Bt proteins lethal to insects and also transfer the gene into chop plants. The procedure is portrayed in number 1.


Figure 1Bt technology.

First, scientists identify a strain of Bt that kills the targeted insect. Climate they isolation the gene the produces the lethal protein. That gene is removed from the Bt bacterium, and a gene conferring resistance to a chemical (usually antibiotic or herbicide) is fastened that will prove beneficial in a later step.

The Bt gene v the resistance gene enclosed is placed into plant cells. In ~ this point, researchers must identify which tree cells have successfully received the Bt gene and also are now transformed. Any kind of plant cell that has the Bt gene must additionally have the resistance gene the was attached to it. Researchers prosper the tree cells in the existence of the antibiotic or herbicide and select the plant cells that are unaffected by it. This genetically changed plant cells are then grown into totality plants by a process called tissue culture. The amendment plants produce the exact same lethal Bt protein created by Bt bacteria since the plants now have the very same gene.

Research to deliver insect resistance genes from Bt to chop plants is fine under way. Corn, cotton and also potatoes space three that the numerous commercial plants targeted because that Bt insect resistance.

Bt in corn confers resistance to europe corn borers

Modern corn hybrids have actually some resistance to europe corn borers (ECB), yet they quiet sustain damages from moderate and high level of infestation. Genetically engineered insect-resistant corn is providing a brand-new defense versus an old enemy.

The enemy

The european corn borer is a significant pest of corn in phibìc America and Europe. Researchers calculation that productivity loss due to ECB averages 5 percent, but any kind of one year or location can have much greater damage (Ciba Seeds). The variety of larvae in the ahead season, the type of winter weather, organic parasites and disease, and also weather problems during the growing season can influence ECB levels. For example, throughout the 1990 and 1991 farming seasons, ECB levels were very high throughout much the the U.S. Corn Belt and losses were heavy. But the cool, merganser weather and also diseases during crucial parts the the 1992 growing season decreased ECB populations and also their damage in countless areas.

ECB larvae attack corn two times during the growing season across most the the U.S. Corn Belt. First-brood larvae feed on plant leaves before flowering, injure sheet tissue, and also eventually bore into the stalk. Second-brood larvae start feeding in the leaf sheath and collar area the corn plants after flowering. Eventually, lock bore into the stalk and also ear shank wherein they can cause damaged stalks and also dropped ears.

The greatest resource of ECB damages may not be visible. ECB disrupts the physiological processes of corn and can alleviate yields even when stalk lodging (breaking) difficulties are no evident. Physiological damage can cause low test weight of harvested corn, little ears, or early fatality of the plant.

Chemical sprays need to be used before the borer tunnels into the stalk to it is in effective. Multiple applications might be required and, even then, complete control is not possible.

The Bt solution

Scientists know that a family of insecticidal protein from Bt death ECB. The bacteria create the protein together an insoluble crystal. Once a susceptible caterpillar, such as the corn borer, eats the crystal, part of it binding to, penetrates and collapses the cells lining that is gut, causing death.

Although Bt genes have actually been introduced right into tobacco, tomatoes, cotton and other broadleaf plants, gene transfer modern technology for corn is a current achievement. The advancement of corn tree expressing Bt protein requires considerable changes in the Bt genes, including the creation of fabricated versions that the genes, quite than the microbial Bt gene itself.

For example, one company has occurred three versions of a man-made Bt gene the "switches" on in pollen, in eco-friendly tissue, or in various other parts of the corn tree (Ciba Seeds). In addition to a herbicide resistance gene, the three Bt versions to be " shot" by a biolistic gene gun right into immature corn embryos taken from arising seeds around 15 work after pollination. The gene pistol bombarded the embryos through tiny gold particles coated v the genes.

Some plants got genes through a non-specific promoter (switch) that led to production the the Bt protein in plenty of parts the the corn plant. Various other plants got a mixture the genes through switches that caused Bt protein production only in the pollen and green tissue. The herbicide resistance gene enabled scientists to determine which tree cells had received a gold fragment carrying DNA and also successfully included the gene. Subsequent screening for Bt production showed which cells received both herbicide resistance and the Bt gene(s).

When the cells were grown into plants and also field tested because that resistance, that was discovered that the Bt plants fared better than any of the regulate plants. In general, plants through both the pollen and green tissue Bt gene appeared an ext resistant to second-brood corn borer damages than plants v Bt genes that expressed us in plenty of parts the the plant. Another benefit of the Bt gene with specific switches (pollen and green tissue) was that they created insecticidal protein in those parts of the plant attacked by both first- and also second-brood ECBs, when minimizing production in seed and also other components of the plant where defense is no critical.

Using Bt modern technology to regulate corn borers is an global effort. One U.S. Firm is using a biolistic gene gun come bombard Indonesian corn through Bt genes that produce proteins lethal to the oriental corn borer (Wilson). This pest is responsible for far-ranging yield losses, approximately 40 percent, in lot of the corn-growing locations of Indonesia.

The warmth Indonesian climate and the life cycle of the eastern corn borer allow 9 to 12 overlapping generations annually, making it a danger to corn essentially year-round. Presenting Bt insect resistance genes right into commercial Indonesian corn appears to be the most feasible and also effective method available to control the pest.

Comprehensive experimentation must it is in done, approval by pertinent authorities obtained, and patent inquiries answered before any company can commercialize corn hybrids containing the Bt gene.

Bt resistance for the Colorado potato beetle

The potato is the most well-known vegetable among North American consumers. It is estimated that potatoes, in part form, space consumed throughout one that every three meals (Monsanto). Regardless of their popularity, potatoes are not that basic to grow. Every year, potato growers shed a far-reaching amount the their crops to pest called the Colorado potato beetle. The beetle feeds ~ above the growing plant leaves and also stems during the growing season, stunting the plant and also cutting yields. Repetitive applications of chemistry sprays are usually necessary to control the pest.

The financial picture

According come the USDA, potato producer in the U.S. Spend $20 million come $40 million annually for control of the Colorado potato beetle (Monsanto). In an mean year, growers spray their areas with insecticides as countless as 5 times in ~ a price of up to $150 every acre.

If insect-resistant plants to be available, researchers estimate that the use of chemistry insecticides in potatoes can be reduced as lot as 30 percent come 40 percent. Interpreted into dollars, growers might save $6 million come $16 million annually, based on USDA estimates of current insecticide use.

Similar science, different target

The scientific research being offered to develop potatoes resistant come the Colorado potato beetle is comparable to the europe corn borer technology. One of the advantages of Bt is the diversity that strains that have the right to be supplied to target various insect pests.

Like the corn researchers, potato scientists are utilizing a specific kind of the naturally occurring soil bacter Bacillus thuringiensis. A solitary gene native the Bt is spliced into a chromosome the a potato cell. As soon as the cell transporting the Bt gene is grown into a plant, the tree produces a protein the is toxicity to the Colorado potato beetle. When the beetle feeds ~ above the genetically improved plant, the toxic protein interferes v its digestive system and it dies.

The protein is highly selective, affecting only the Colorado potato beetle. It does not harm humans, animals or valuable insects that aid control other crop pests.

The outlook

One firm conducting insect-resistant potato study expects farmers to be planting your beetle-resistant potato by the mid-1990s (Monsanto news release). This company began its advance of insect-resistant potatoes with the Russet Burbank variety, because it comprises approximately 40 percent that U.S. Potato acreage. However, firm researchers are likewise working to boost other renowned potato arrays such together Atlantic, Superior, Norchip and Shepody.

Like every genetically improved food crops, the exact timetable for commercialization the insect-resistant potatoes depends not just on the clinical tests that must be conducted, but likewise on approvals by the proper regulatory agencies. (See regulating Bt plants.)

Bt resistance because that cotton

Cotton is an additional cash crop benefiting indigenous Bt insect-resistance research. Through a industry value the $4.5 billion, cotton is the nation"s 5th largest chop (Monsanto). However annually, noodle growers suppose to shed up to 15 percent of each field because of insect damage.

Caterpillar insects, such as the tobacco budworm, cotton bollworm and pink bollworm, feed on noodle plants. Professionals say the these and similar pests room responsible for numerous dollars in damage to the American cotton crop each year.

Caterpillar economics

Caterpillar insects room responsible because that 60 to 70 percent of every insect damages to noodle plants (Monsanto). Initiatives to regulate them chemically account for about 60 come 70 percent of a cotton grower"s pesticide costs. In the Mississippi Delta growing region, a cotton grower"s pesticide expenses can typical $40 to $45 every acre each season. In Arizona, pest control costs are usually higher, perhaps twin that of the Mississippi Delta.

Chemical sprays include Bt are seldom handy in cotton, because Bt breaks under in sunlight, washes far in rain, and also is almost impossible to use to the plant components where insects feed. However, various other chemical insecticides are used that may need to be used as countless as 10 times in one season. Through planting insect-resistant cotton, researcher hope farmers have the right to reduce the variety of insecticide applications they have to pay for.

Same song, 3rd verse

Creating insect resistance in cotton by making use of Bt technology is a procedure comparable to that supplied in corn or potatoes. Researchers discover one or an ext strains the Bt that create a protein deadly to some of the many damaging cotton insect pests. The gene that regulates the protein"s production is inserted into the hereditary structure of cells from a cotton plant. The cell that efficiently receive the Bt genes space grown into totality plants with tissue culture and go into a long duration of laboratory and field testing. When specific caterpillar pests feed on the new cotton plants, the protein loss their cradle systems, and they die.

Commercialization timetable

Current estimates are the it will be several years prior to insect-resistant noodle plants room commercially marketed. Castle must fulfill the regulatory demands administered through the USDA and the eco-friendly Protection firm (EPA). (See regulation Bt plants.)

The insect resistance problem

Transgenic Bt tree such as corn, potatoes and also cotton have actually a feasible problem — what if insects develop up a resistance come the lethal proteins?

The potential that pests to build resistance versus the defense mechanisms of plants is well-known and also is not unique to gene engineered plants. Insects may build resistance to a chop defense no matter just how it to be developed. The chop defense can be a chemical or organic agent, a gene currently in the crop types and moved to commercial plants by standard plant-breeding methods, or a gene presented by recombinant DNA technology. Because much more than 500 insects and also mites already have gained resistance to a variety of insecticides, over there is worry that comparable resistance come Bt toxins could develop (McGaughey and Whalon).

Several major pests, consisting of the tobacco budworm, Colorado potato beetle, Indianmeal moth and also diamondback moth, have demonstrated the ability to it is adapted to Bt in the laboratory. It has actually been reported the the diamondback moth evolved high levels of resistance in the ar as a result of repetitive use the Bt (McGaughey and Whalon). Together Bt use boosts on an ext acres, some scientists predict the insect resistance come Bt will certainly be a significant problem. Considerable controversy exists around how Bt must be controlled to prolong its usefulness.

Bt durability in non-transgenic crops

The decision proteins that Bt that space lethal come insects have actually been widely offered as microbial insecticides in timeless corn and also vegetable plants for years. In recent years, field populations that insects tolerant to this Bt proteins have actually been reported. Come date, these situations are restricted and have actually been linked with frequent and lengthy use that Bt top top geographically diverted insect populations (Ciba Seeds). Scientists have been able come induce higher levels the resistance in these field populations by acquisition them right into the laboratory and also exposing lock to particular Bt insecticidal decision proteins.

A laboratory population of tobacco budworm that is resistant to more than one course of crystal proteins has been reported. However, the resistance the this populace is not high, and its capability to make it through is substantially reduced when the proteins space absent.

In the case of ECB-resistant corn, that is believed that the development of insect resistance might be delay by the accessibility of non-Bt corn fields (refuges) that room still susceptible to the borers. Borers that could not make it through ECB-resistant areas may do well in the refuges. Over there they might breed with resistant borers, diluting the resistance properties in the gene pool.

Bt to trust in transgenic crops

About 13 U.S. And also European suppliers that fund scholastic Bt study have formed a Bt management Working group to support research study on the interaction of transgenic plants v target pests.

The plant sector is employing several approaches to deal with insect resistance to transgenic insecticidal plants, including the following:

Very high levels of insecticidal decision proteins (ICPs) room being created in brand-new genetically modification plants. The just insects that have the right to survive feeding on together plants will certainly be those that currently possess a high-level resistance gene as component of their hereditary code. Together genes are expected come be extremely rare in the insect population. Insects with much more common genes for partial, or low-level, resistance will not endure the an extremely high level of ICPs.

Additional insecticidal genes room being sought. The idea is to develop transgenic plants the express numerous insecticidal gene targeting different sites in ~ the insect. In order to make it through plants v such many defenses, an insect would probably have to possess many resistance genes. Part scientists think it is highly unlikely the insects can gain such genes. In any kind of case, these scientists anticipate the plants expressing added insecticidal genes will certainly be available before insect resistance to a single, extremely expressed ICP from Bt develops.

Regulating Bt plants

Insect-resistant crops occurred through Bt modern technology are topic to the same regulations as other genetically engineered plants. It should be noted that U.S. Regulatory plan for gene engineered plants has evolved over time and added changes in the demands discussed listed below may occur. Three federal government agencies share obligation for administering plant biotechnology regulations in the joined States.

U.S. Room of farming (USDA)

The USDA administers the commonwealth Plant insect Act (FPPA), i beg your pardon regulates interstate movement, importation, and also field trial and error of gene engineered plants. A unique permit native the USDA and approval by the individual state departments of agriculture are compelled to move any genetically engineered organism right into the U.S. Or in between states. Applicants for a allow must administer details around the nature that the organism, that is origin, and also its plan use. If granted, the permit certifies the the applicant"s facility and also procedures meet specific operating standards, together as suitable levels the containment to avoid accidental to escape of the organism. Guidelines collection by the nationwide Institutes of health and wellness for laboratory research including recombinant DNA molecule are consisted of in this standards and must be followed by the applicant.

The USDA oversees field testing of genetically engineered crops. The applicant must carry out complete information around the plant, consisting of all new genes and brand-new gene products, their origin, the function of the test, the experimental design (how the test will be conducted), and precautions to be taken to prevent the escape of pollen, tree or plant parts from the field test site. The approval process can take it a preferably of 4 months.

Before a gene engineered chop can be marketed commercially, a petition have to be filed for USDA exemption. This petition requires an ext information 보다 a field test permit, including eco-friendly product safety and security information.

Recently, the USDA systematized the permit procedure for movement and also field testing of certain genetically engineered plants such as corn, soybeans, cotton, potatoes, tomatoes and also tobacco. The streamlining was based on an extensive background of safe use in field trials in the unified States. In the systematized process, one applicant can ar test details crops if the crop and also genetic product meet details USDA power standards. For a field test the does not meet these power standards, the current 120-day field test permit procedure would be followed.

Environmental protection firm (EPA)

The EPA freshly proposed new policies for evaluation of applications for field trials of gene engineered plants. It proposed that substances presented into plants by genetic engineering to provide condition or insect resistance (Bt plants are in this category) autumn under the commonwealth Insecticide, Fungicide, and Rodenticide Act and also the federal Food, Drug, and also Cosmetic Act.

To date, the USDA has actually consulted through EPA when reviewing applications to test such plants on a little scale. The EPA will most most likely formally review a product when a company decides to move right into large-scale testing. Similar to traditional pesticides, an speculative Use permit (EUP) will certainly be compelled for tests the exceed 10 acres complete in the U.S.

After receiving the EUP, a company follows a procedure that entails extensive experimentation of the chop to ensure food and also environmental safety before the crop is commercialized. In addition, the is likely that part applicants additionally will consult the Food and Drug administration (FDA) before a pest-resistant food crop is commercialized, even though a recent FDA plan proposal emphasized that such commodities fall under the EPA"s jurisdiction and also are not subject to FDA pre-market approval.

Food and also Drug administration (FDA)

The FDA additionally has really broad authority under the commonwealth Food, Drug, and Cosmetic act to manage the arrival of brand-new food crops, even if it is they were conventionally produced or developed by genetic engineering. FDA plan states the any new food developed through genetic change may require experimentation and/or labeling if the hereditary material introduced right into the host plant originates from outside the classic food supply, is a well-known or suspected cause of allergy reactions, or significantly alters the nutritional composition of the food (Federal Register). Every agency or individual that produces food or food assets through recombinant DNA an innovation is legally forced to guarantee its safety and quality before it beginning the food supply — the same assurance forced of timeless food products.

The Bt outlook

Crops that have been genetically engineered for Bt resistance could dramatically reduced production costs and provide farmers with new insect control alternatives within the next few years. The success of their commercialization depends on several factors, consisting of the regulatory climate, patent issues, and also the ability of scientists to address targeted insects that develop resistance come the lethal proteins. Number of companies currently working top top Bt insect resistance in crops foresee marketing their assets in the mid-1990s.


Ciba Seeds. "Ciba seeds Introduces Gene;" "New Agreement between INRA, CIBA for studies on Insect-Tolerant Corn;" "Plant Breeding an innovation and DNA Markers;" "Questions and also Answers — benefits of Insect-resistant Corn because that Farmers;" "Strategy for controlling Insect Resistance come Transgenic Bt Corn;" "U.S. Federal government Regulation of plant Biotechnology" — News releases. "Ciba"s Bt Maize Work" — summarized article. Ciba Seeds, P.O. Crate 18300, Greensboro, phibìc Carolina.Federal Register, "Statement of Policy: Foods derived from brand-new Plant Varieties; Notice." part IX, room of Health and Human Services, Food and Drug Administration. Commonwealth Register, volume 57, number 104, Friday, may 29, 1992. P. 22984-23005.Gould, Fred. "Evolutionary Biology and Genetically Engineered Crops." Bioscience. Volume 38, number 1, January 1988. P. 26-33.Gould, Fred; Martinez-Ramirez, Amparo; Anderson, Arne; Ferre, Juan; Silva, Francisco J.; and also Moar, william J. "Broad-spectrum Resistance to Bacillus thuringiensis toxin in Heliothis uirescens." Proceedings the the national Academy of Science, USA. Volume 89, September 1992. P. 7986-7990.Marois, James J.; Grieshop, James I.; and also Butler, L. J. (Bees). "Environmental Risks and Benefits of farming Biotechnology." In Agricultural Biotechnology, Issues and Choices, p. 67-79. Edited by bill R. Baumgardt and also Marshall A. Martin. West Lafayette, Indiana: Purdue University agricultural Experiment Station, 1991.McGaughey, wilhelm H. And also Whalon, mark E. "Managing Insect Resistance come Bacillus thuringiensis Toxins." Science.

See more: When The Emperor Was Divine Shmoop, When The Emperor Was Divine Summary

volume 258, Nov. 27, 1992. P. 1451-1455.Monsanto. "B.T. — The within Story;" "Insect-Resistant noodle Replaces Insecticides;" "Self Defense because that Potatoes" — News releases. The agricultural Group, publicly Affairs Department, 700 Chesterfield Parkway North, St. Louis,, H.M. An individual communication. ICI Seeds, Slater, Iowa, march 30, 1993.Wrage, Karol. "Bt"s: Flagship because that 21st Century BioControls: market Execs, college Researchers to speak Future Promising and also Profitable." Biotech Reporter, volume 10, number 5, might 1993. P. 8-9.