Nutrient Agar is a basic purpose, nutrient tool used because that the growing of microbes supporting expansion of a wide variety of non-fastidious organisms. Nutrient agar is popular due to the fact that it can prosper a variety of varieties of bacteria and also fungi, and also contains plenty of nutrients required for the bacterial growth.

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Composition the Nutrient Agar

0.5% Peptone

It is one enzymatic digest of pet protein. Peptone is the principal resource of necessary nitrogen for the growing bacteria.

0.3% beef extract/yeast extract

It is the water-soluble substances which aid in bacterial growth, such together vitamins, carbohydrates, essential nitrogen compounds and also salts.

1.5% agar

It is the solidifying agent.

0.5% NaCl

The visibility of sodium chloride in nutrient agar maintains a salt concentration in the medium that is similar to the cytoplasm that the microorganisms.

Distilled water

Water is essential for the growth of and reproduction of micro-organisms and also provides the medium through which miscellaneous nutrients deserve to be transported.

pH is changed to neutral (7.4) at 25 °C.

Preparation that Nutrient Agar1. Suspend 28 g the nutrient agar powder in 1 litre the distilled water.

2. Heat this mixture when stirring to completely dissolve all components.

3. Autoclave the dissolved mixture at 121 degrees Celsius because that 15 minutes.

4. Once the nutrient agar has been autoclaved, enable it to cool however not solidify.

5. Pour nutrient agar right into each plate and leave bowl on the sterile surface until the agar has actually solidified.

6. Replace the lid of each Petri dish and also store the bowl in a refrigerator.

Uses of nutrient Agar1. It is frequently used because that isolation and also purification of cultures.

2. The can additionally be provided as a way for creating the bacter lawns needed for antibiotic sensitivity tests. In actuality, antibiotic sensitivity experimentation is commonly performed top top media specially formulated for that purpose.

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Four nutrient agar key growing swarms of common Gram negative bacteria.Source: Wikipedia
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